Determination of Organic Acids in Apple Juice by Capillary Liquid Chromatography
Authors:
D. Blanco a;
M. E. Quintanilla a;
J. J. Mangas b;
M. D. Gutierrez a
| Affiliations: | a Departamento de Qu mica F sica y Anal tica, Universidad de Oviedo, Oviedo, Spain |
b Centro de Investigaci n Aplicada y Tecnolog a Agroalimentaria, Asturias, Spain |
DOI:
10.1080/10826079608014042
Publication Frequency:
20 issues per year
Published in:
Journal of Liquid Chromatography & Related Technologies,
Volume
19,
Issue
16
September
1996
, pages 2615
- 2621
Subject:
Chromatography;
Formats available:
PDF
(English)
View Article:
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Abstract
The organic acids quinic, malic, shikimic and citric were separated on a packed reverse phase fused-silica capillary column using 0.01 M K2HPO4/H3PO4 at pH 2.7 as eluent at a flow rate of 2 μL/min, and determined with UV detection. Conventional liquid chromatographicequipment was adapted for such purposes.
The organic acids were able to be separated with detection limits of 2.4, 2.1, 0.04 and 2.9 ng, respectively. Application of the proposed method to the quantification of organic acids in apple juice is reported. |
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