In this study, the mouse neuroblastoma cell line Neuro-2a was analyzed for expression of angiotensin II receptors. Reverse-transcriptase polymerase chain reaction (RT-PCR) showed that Neuro-2a cells express mRNA of angiotensin II (AngII) receptor subtypes AT_1A, AT_1B, and AT₂. Analysis of Neuro-2a cells by Western blotting revealed AT₁ and AT₂ receptor protein expression. The predominant molecular weights were determined to be 50.4 kDa for the AT₁ receptor and 62.4 kDa for the AT₂ receptor. Observation of AT₁ and AT₂ receptor localization within Neuro-2a cells using immunocytochemistry showed distribution similar to other G-protein coupled receptors with diffuse distribution in the cytosol, perinuclear enrichment and accumulation of receptors on the outer cellular periphery with extension into the neurites. Furthermore, we observed InsP₃ formation following AngII induction that could be abolished in presence of the AT_1A receptor antagonist losartan. The results clearly show expression of the AngII receptor types AT_1A and AT₂ in the Neuro-2a cell line. We conclude that Neuro-2a cells represent an interesting model cell line for study of mechanisms that control the interplay between these receptors.