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Partial Purification of Anthocyanins from Brassica oleracea (Red Cabbage) 

Authors: M. R. Coutinho a;  M. B. Quadri a;  R. F. P. M. Moreira a; M. G. N. Quadri a
Affiliation:   a Department of Chemical and Food Engineering, Federal University of Santa Catarina, Campus Universitaacuterio, Trindade, Florianoacutepolis, SC, Brazil
DOI: 10.1081/SS-200036539
Publication Frequency: 16 issues per year
Published in: journal Separation Science and Technology, Volume 39, Issue 16 January 2004 , pages 3769 - 3782
Formats available: HTML (English)
Previously published as: Separation Science (0037-2366) until 1978
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Abstract

Red cabbage has a high content of anthocyanin and it may become an important source of this pigment. Common extraction methods are not selective, and a great quantity of sugar is co-extracted with the dye. This work used adsorption-desorption process on Amberlite XAD7 and Sephadex LH20 resins to partially purify anthocyanins present in the whole juice of red cabbage. Both resins succeeded to purify anthocyanins, producing solutions with up to 0.5 g L-1 of sugars from an initial concentration of more than 25 g L-1. Using Amberlite XAD7 it was possible to recover 24 to 95% of the dye initially found in the whole juice while 11 to 56% was recovered using Sephadex LH20. The kinetics of adsorption of the dye on Amberlite XAD7 was described according to a pseudo-second-order model, and the sorption equilibrium was well described by the Freundlich isotherm. The adsorption enthalpy was -17.13 kcal mol-1, indicating a possible chemical adsorption.
Keywords: Anthocyanin; Purification; Sorption; Red cabbage; Kinetics
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