We aimed to optimize germination and growth conditions of the arsenic hyperaccumulating fern, Pteris vittata L. Pot experiments were carried out to investigate the effects of soil pH, soil calcium (Ca) concentration, and temperature on the sexual propagation of P. vittata. At 25°C, germination was both accelerated and increased by high soil pH and Ca concentration. Spores of P. vittata did not germinate on medium with a pH of 4.6. Amending strongly acid soils with 27.5 or 40 μmol/g Ca(OH)₂ significantly improved the growth rate during both the germination phase and the gametophyte phase. Amending strongly acid soils with NaOH (55 μmol/g) promoted germination, but did not affect subsequent growth. Among the different temperature, germination and growth rates were higher at 25°C than at 20°C or 30°C. The distribution of P. vittata in China might be influenced by its requirement for high pH and high Ca concentration in the soil, and appropriate growth temperature to complete sexual propagation. These results provided important information for improving breeding conditions of P. vitatta and will be helpful for extending the range of areas in which P. vittata can be used for phytoremediation.