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Influence of Process Conditions During Impulsed Electrostatic Droplet Formation on Size Distribution of Hydrogel Beads 

Authors: Dorota Lewinacuteska a;  Stefan Rosinacuteski a; Andrzej Werynacuteski a
Affiliation:   a Institute of Biocybernetics and Biomedical Engineering, Warsaw, Poland
DOI: 10.1081/BIO-120028667
Publication Frequency: 8 issues per year
Published in: journal Artificial Cells, Blood Substitutes, and Biotechnology, Volume 32, Issue 1 June 2004 , pages 41 - 53
Formats available: HTML (English) : PDF (English)
Previously published as: Artificial Cells, Blood Substitutes, and Immobilization Biotechnology (1073-1199, 1532-4184) until 2003
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Abstract

In the medical applications of microencapsulation of living cells there are strict requirements concerning the high size uniformity and the optimal diameter, the latter dependent on the kind of therapeutic application, of manufactured gel beads. The possibility of manufacturing small size gel bead samples (diameter 300 µm and below) with a low size dispersion (less than 10%), using an impulsed voltage droplet generator, was examined in this work. The main topic was the investigation of the influence of values of electric parameters (voltage U, impulse time τ and impulse frequency f)on the quality of obtained droplets. It was concluded that, owing to the implementation of the impulse mode and regulation of τ and f values, it is possible to work in a controlled manner in the jet flow regime (U> critical voltage UC). It is also possible to obtain uniform bead samples with the average diameter, deff, significantly lower than the nozzle inner diameter dI (bead diameters 0.12-0.25 mm by dI equal to 0.3 mm, size dispersion 5-7%). Alterations of the physical parameters of the process (polymer solution physico-chemical properties, flow rate, distance between nozzle and gellifying bath) enable one to manufacture uniform gel beads in the wide range of diameters using a single nozzle.
Keywords: Alginate; Microencapsulation; Electrostatic droplet formation; Impulsed mode
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