The uptake equilibrium and the column dynamics of alanine by “0017” resin, a strongly acidic cation exchange resin, were investigated. The experimental data indicated that the Langmuir equation could describe the equilibrium isotherm. The implicit difference scheme and the optimization technique were applied to compute the model developed for alanine in fixed-bed column. The results of experiments with different superficial velocities and liquid phase solute concentrations showed that interparticle transport was dominated by the slow diffusion of alanine cations through the liquid phase bulk into the surface of the resin particle. However, the resistance of intraparticle diffusion of alanine cations through the macroreticular polymer structure of the resin was small. The experimental results were in good agreement with the theoretical predictions.