FERMENTATION OF CHEESE WHEY IN AN IMMOBILIZED-CELL FLUIDIZED-BED REACTOR
Authors:
A.S. Bassi a;
S. Rohani a;
D.G. MacDonald a
| Affiliation: | a Department of Chemical Engineering, University of Saskatchewan, Saskatoon, Canada |
DOI:
10.1080/00986449108910866
Publication Frequency:
12 issues per year
Subjects:
Biochemical Engineering;
Chemical Engineering;
Combustion;
Fluid Mechanics;
Reaction Engineering;
Semiconductors;
Formats available:
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(English)
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Abstract
Batch and continuous fermentation of cheese whey to lactic acid were carried out in a fluidized bed reactor by using Lactobacillus bulgaricus immobilized in 3% (w/v)garose. Five batch fermentations conducted under uncontrolled pH conditions and initial lactose concentrations ranging from 40 to 60g/L gave an average final lactic acid concentration of 6·9 g/L in a 48 to 50 h period. Continuous fermentation under controlled pH conditions at four different dilution rates ranging from 0·043 to 0·428 h-1 and a lactose inlet concentration of 43·5 g/L resulted in a lactic acid yield of 0·26mg/mg lactose consumed. The maximum lactic acid concentration was 10·0 mg/mL at a dilution rate of 0·086 h-1 with a substrate utilization of 90·5∥. The mathematical model of the system for batch and continuous operations was developed and the model parameters were determined experimentally and by non-linear multiple regression analysis.
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| Keywords: Cheese whey fermentation; Lactic acid; Fluidized-bed; Immobilized-cell Batch and continuous cultures |
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