This study explored connections between media use and college students' attitudes of acceptance towards homosexuality (AATH). Data were collected from 1,761 undergraduates (62.7% female, M_Age = 19 years). Results varied by the gender, ethnicity, and religiosity of the participants. Overall, greater media consumption among men and those who are highly religious was associated with greater AATH, whereas the reverse was true among women and those who are less religious. Although the associations were modest, the results support evidence of a mainstreaming effect, whereby increased media exposure may draw groups with disparate attitudes towards a more similar viewpoint on homosexuality.